Ooooh, I thought it meant K Rool or Isabelle or someone had canon sexual intercourse.
Although, I guess Simon did? And Ridley...? How are little birdies born?
Oh, yeah! For the newcomers.
*AHEM*
Inklings: Ew. They're kid-squids. Granted, Toon Link got an honorary mention on the original list as he canonically
will reproduce but had yet to do so at the time in his life represented in Smash, but... No.
Ridley: Between being the last of his kind & being canonically too obsessed with himself to seek a mate, I'm going to say no.
Daisy: My old roommate was insistent that her holding Luigi's hand on Daisy Circuit means they've had intercourse because Japanese cultural stuff. I mostly pushed back because I couldn't imagine Luigi having sex, but that shouldn't stop Daisy. I'll give it a "maybe."
Simon: Definitely yes at some point ala Toon Link above, although with his Smash incarnation, I'm just going to give him a "probably." If his Smash self is based on Castlevania 1, if Haunted Castle was a remake of Castlevania 1, if Haunted Castle took place on Simon's wedding
day, & if Simon's holy enough to use holy water & therefore would wait until his wedding
night, there's a chance he's still celibate until after he kills Dracula.
Richter: Second verse, same as the first, barring the Haunted Castle stuff.
Dark Samus: Not sexually active; she'd have no biological need nor drive.
Chrom: Absolutely sexually active, with Lucina being Exhibit A.
King K. Rool: Inconclusive, but he claims to have a wife in DKC3. Is it true? Is it a joke? Who knows? But I don't see any reason why he wouldn't be sexually active. I just don't see any proof, either.
Isabelle: Inconclusive, but she seems too innocent.
... The downside to this list is that there are few characters with proof of a sex life or lack thereof, & the majority of the ones with proof are solely because they're parents (The list was inspired by a 2 AM musing that Olimar was one of the few characters with kids in Brawl, & therefore was one of the only sexually active characters).
And yes, Goku would immediately be on the "has had sex" list, even if he has apparently never kissed his wife & mother of his 2 sons.