Gwen Stacy - Esentially Spider-man's Lois Lane, Spider-man's intended girlfriend who he dated in college. Her death changed comic books forever
Mary Jane Watson -starting off as a running gag, then as the party girl she developed into Peter's main love interest. Eventually married Spider-man until Joe Queseda's mid life crisis caused Spider-man to sell his marriage to Satan
Black Cat - A cat burglar who Spider-man falls in love with, but differentiates herself from Catwoman by being more obsessed with adventure. She's off and on again about whether or not she's a hero or a villain.
Carlie Cooper = Joe Queseda's midlife crisis peaks when he conceives of Carlie Cooper named after his daughter. Yes, Joe Queseda wanted Spider-man to marry his daughter. Eventually dropped because she wasn't Gwen Stacy, Mary Jane or Black Cat
Cindy Moon/Silk - Bitten by the same radioactive Spider as Peter, this apparently causes them to make out constantly, the pheromone angle was eventually dropped once everyone not named Dan Slott saw how creepy it was and Cindy Moon became a successful character under different writers
There's a few others but none of them are that interesting or went far